Fig. 5

IL-1R1 expression in the IL-1R1-3HA-IRES-tdTomato knock-in line of mice. Vertical sections of the retina were labeled with antibodies to RFP (red; a-d), CD31 (green; b), S100β (blue; b), Otx2 (green; c), Sox9 (blue; c and d), and Vsx2 (green; d). Retinas were obtained from control, saline-treated eyes (a, b, and e), and eyes injected with NMDA (c, d, and e) or IL1β (e). Retinas were harvested at 3 h (c) and 48 h (d) after NMDA-treatment. The histogram in e illustrates the mean, standard deviation, and individual data points for each experimental condition. Significance of difference (p < 0.05) across the treatment groups for each cell type was determined by one-way ANOVA followed by Tukey’s test. Yellow arrows in b indicate RFP⁺ endothelial cells. White arrows in b indicate RFP⁺ astrocytes. Solid arrows in c indicate RFP⁺ bipolar cells. Open arrow heads in c indicate RFP⁺ astrocytes. Double-arrows in d indicate RFP⁺ bipolar cells, and single bold arrows in d indicate RFP⁺ Müller glia. The calibration bars panels a, c and d represent 50 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; RFP, red fluorescent protein