Fig. 6

IL1β stimulates the reactivity and proliferation of microglia in the retina and conveys neuroprotection following NMDA-treatment. Eyes were injected with saline or IL1β, and retinas harvested 24 h later (a–e). Eyes were injected with saline or IL1β on day 1, NMDA on day 2, and retinas harvested 1 or 12 days later (f-m). Retinal sections were labeled with TUNEL (f) or DRAQ5 (red; k) or antibodies to F4/80 (green) and Iba1 (red; a), tomato lectin (green; d) and Ki67 (red; d), calretinin (green; k), or F4/80 (green) and Iba1 (red; l). The box plots in b, c, e, g–j, and m illustrate the mean, upper extreme, lower extreme, upper quartile, and lower quartile (n ≥ 6 animals). Significance of difference (*p < 0.05) was determined by using a t test. Arrows indicate microglia. The calibration bars in panels a, d, f, k, and l represents 50 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer