Fig. 8

Cell death in IL-1R1-null and GFAPCre-IL-1R1r/r. Eyes were injected with saline or IL1β on day 1, NMDA on day 2, and retinas harvested 1 day later. Experiments were performed on wild-type (WT), IL-1R1-null, andGFAPCre-IL-1R1r/r. The histograms illustrate the mean (± SD and individual data points) number of dying cells across all layers of the retina (a), in the INL (b), and in the GCL (c). Retinal sections were labeled using the TUNEL method (d) or antibodies to RFP (red) and S100β or Sox9 (green) (e). Significance of difference (p < 0.0001) among the treatment groups was determined by one-way ANOVA. Significance of difference (*p < 0.05) between treatment groups was determined using a Tukey’s multiple comparison test. The calibration bars in panels d and e represent 50 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer