Fig. 4

Clenbuterol administration after stroke onset increases infarct size. a Quantification of infarct size (normalized to contralateral hemisphere) at 3 days, Student’s t test (n = 10 mice per group) and representative images of brain sections stained with NeuN and Cresyl Violet showing the anterior-to-posterior spread of the infarcted region at 3 days after stroke in mice treated with saline or clenbuterol. Clenbuterol was given as a 1 mg/kg i.p. bolus injection and 1 mg/kg/day via subcutaneous pump. b Quantification of systolic blood pressure and c heart rate after a single i.p. injection of 1 mg/kg clenbuterol or saline, two-way repeated measures ANOVA with Sidak’s multiple comparisons test (n = 10 mice per group). d Performance on the rotating beam task represented as percent of baseline distance traveled (average of four trials). e Performance on the foot fault gridwalk task, represented as correctly placed steps as percentage of total steps counted (n = 12 mice per group). For behavioral testing, mice were treated with a single 1 mg/kg injection of clenbuterol per day for the first 7 days after stroke. Bars and points, mean ± SEM *p < 0.05, **p < 0.01, ***p < 0.001