Fig. 3

Knockdown of LXRβ expression in ACC by shLXRβ blocked GW3965-mediated analgesic effects in CFA-injected mice. a The schematic showed the experimental design for virus injection, drug treatment, and behavior tests. b, c LXRα knockdown by shLXRα did not affect GW-mediated analgesia of mechanical allodynia induced by CFA. d, e shLXRβ abolished GW-mediated analgesia of mechanical allodynia induced by CFA. f, g shLXRα did not affect GW-mediated analgesia of thermal hyperalgesia induced by CFA. h, i LXRβ knockdown by shLXRβ abolished GW-mediated analgesia of thermal hyperalgesia induced by CFA. Error bars represent SEM. n = 6, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. shNC-injected group; ^#p < 0.05, ^##p < 0.01 vs. shNC + CFA group; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001 vs. shLXRα + CFA group