Fig. 4

GW3965 inhibited the increased nuclear translocation of NF-κB and MAPK phosphorylation in mice ACC after CFA injection. a, b Co-IP revealed the interaction of endogenous LXRβ with NF-κB p65 in mouse ACC. c Representative images of nuclear translocation of NF-κB p65 in ACC from Sham, and mice after CFA injection with or without GW3965 administration (GW, 10 mg/kg) were stained for p65 (red) and Hoechst (blue). d, e The time course of p65 nuclear translocation in ACC after CFA insult. GW treatment (10 mg/kg) reversed the enhanced nuclear translocation of p65 (f, g), p50 (h, i), and p-IκBα expression (j, k) after CFA injection. n = 5, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. Sham; ^#p < 0.05, ^###p < 0.001 vs. CFA-injected group