Fig. 6

GW3965 infusion abolished the enhanced excitatory transmission in CFA-injected mice. a Representative traces showed averages of EPSCs at 10 and 30 V stimulation intensity in mice ACC. b Plot of input-output curves showed GW3965 (GW, 10 μM) perfusion partially abolished the enhancement of synaptic transmission in ACC of CFA-injected mice. n = 6 neurons/3 mice. c Representative mEPSCs recorded in pyramidal neurons of ACC at a holding potential of − 70 mV. d Cumulative frequency (left) and amplitude (right) histogram of the mEPSCs from the cells in each group. e Quantitative analysis of neurons in ACC from each group showed GW incubation blocked the enhanced mEPSC frequency (left) induced by CFA, while GW had no effect on mEPSC amplitude (right). n = 7 neurons/3 mice in control, n = 9 neurons/3 mice in CFA + vehicle, and n = 9 neurons/3 mice in CFA + GW mice. f GW treatment (10 mg/kg) reversed enhanced phosphorylation of GluR1 at g Ser831 (p-GluR1-S831) and h Ser845 (pGluR1-S845) induced by CFA injection. Error bars represent SEM. n = 6, ^**p < 0.01, ^***p < 0.001 vs. Sham group, ^#p < 0.05, ^##p < 0.01 vs. CFA-injected group