Fig. 1

Increased immunoreactivity of sEH in activated astrocytes occurred in parallel with the progression of AD. Representative confocal images show that sEH immunoreactivity (green) was found within activated astrocytes (red) in the vicinity of Aβ plaques (blue) in APP/PS1 mice at the age of 14 months, whereas GFAP-positive astrocytes with less activated morphology showed minimal immunoreactivity for sEH. In age-matched Wt littermates, GFAP-positive astrocytes were observed with resting morphology and contained little immunoreactivity for sEH. In the right panel, the semi-quantification of the total intensity of sEH immunoreactivity centered by Aβ plaques with a diameter of 200 μm in APP/PS1 mice at 5 months (5m, n = 5), 9 months (9m, n = 10), and 14 months (14m, n = 11) of age is shown. The data indicate that the levels of sEH immunoreactivity escalated with aging and in parallel with the progression of astrocyte activation in APP/PS1 transgenic mice. Data are presented as the mean ± SEM. One-way ANOVA and Bonferroni multiple comparison test were performed. *p < 0.05, **p < 0.01, ***p < 0.001