Fig. 3

The effects of sEH expression in primary astrocytes on the mRNA levels of LPS-induced pro-inflammatory markers. LPS activated primary astrocytes, as evidenced by significant increases in the mRNA levels of iNOS and COX-2 in Wt, si-scrambled, and plasmid controls (a). Manipulations of astrocytic sEH levels using si-sEH (n = 9) or sEH^−/− (n = 6) led to further increases in iNOS (p < 0.05 for si-sEH and p < 0.001 for sEH^−/−) and COX-2 (p < 0.001 for both) mRNA levels, but the mRNA levels were slightly reduced in over-sEH (n = 5). Similarly, the mRNA levels of TNFα and IL-6 in primary astrocytes with either si-sEH or sEH^−/− were increased (p < 0.001 for both) compared to respective controls, and over-sEH had no effects on the mRNA levels (b). Data are presented as the mean ± SEM. One-way ANOVA and Bonferroni multiple comparison test were performed. *p < 0.05; **p < 0.01; ***p < 0.001