Fig. 2

Comparison of IDO mRNA and protein expression between study groups. The LPS + GM-CSF group were pretreated with GM-CSF (30 μg kg, i.p.) 30 min prior to LPS injection (0.83 mg/kg, i.p.). Statistical significance was analyzed using one-way ANOVA followed by pairwise comparisons with the Tukey post hoc test and is depicted in both graphs as *p < .05, **p < .01, and ***p < .001. a The bands observed for each individual mouse in the western blot analysis of IDO. b IDO mRNA expression was significantly different between the three groups (p < .001). All pairwise comparisons were significant (control/LPS: p < .001; control/GM-CSF: p = .003; LPS/GM-CSF: p = .032). c IDO protein expression was significantly different between the three groups (p < .001). LPS significantly increased IDO expression (p = .005) while GM-CSF pretreatment inhibited this increase significantly (p = .016)