Fig. 3

Immunohistochemical validation of increased Kv1.3 protein expression in microglia following tMCAO. a Immunofluorescence micrographs from tMCAO brain sections (48 h post-tMCAO) showing microglia/macrophage marker Iba1 (red) as well as Kv1.3 channel expression (green). Minimal Kv1.3 immunoreactivity was noted in the contralateral hemisphere while Kv1.3 positive Iba1⁺ microglia were seen in the peri-infarct region in the ipsilateral hemisphere. Co-localization between Iba1 and Kv1.3 is shown in yellow in the merged image. High-power (60×) images are shown on the right. Red—Iba1; green—Kv1.3; blue—DAPI. Also see Additional file 4: Figure S4 for related imaging from other timepoints. b Immunofluorescence micrographs from tMCAO brain sections (48 h post-tMCAO) showing abundant Tmem119 expression by ramified microglia in contralateral hemisphere while ipsilateral Tmem119 expression is significantly reduced. Decreased Tmem119 expression was associated with increased Kv1.3 expression. Co-localization between Iba1 and Kv1.3 is shown in yellow in the merged image. High-power (60×) images are shown on the right. Red—Tmem119; green—Kv1.3; blue—DAPI