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Table 1 Primer sequences and amplicon sizes of genes validated by Taqman RT-PCR

From: Light-emitting diode therapy protects against ventricular arrhythmias by neuro-immune modulation in myocardial ischemia and reperfusion rat model

Gene name Accession no. Primer sequence Amplicon size, bp
IL-1β NM_031512.2 F: 5′-TGACCTGTTCTTTGAGGCTGAC-3′
R: 5′-CATCATCCCACGAGTCACAGAG-3′
272
IL-18 NM_019165.1 F: 5′-TCAGACCACTTTGGCAGACTTC-3′
R: 5′-GATTCGTTGGCTGTTCGGTC-3′
134
IL-6 R-IL6(RZ)-S
R-IL6(RZ)-A
F: 5′-AGGATACCACCCACAACAGACC-3′
R: 5′-TTGCCATTGCACAACTCTTTTC-3′
109
TNF-α R-TNF-α-S
R-TNF-α-A
F: 5′-CCAGGTTCTCTTCAAGGGACAA-3′
R: 5′-GGTATGAAATGGCAAATCGGCT-3′
80
GAPDH NM_017008.4 F: 5′-TCAGACCACTTTGGCAGACTTC-3′
R: 5′-TCAGACCACTTTGGCAGACTTC-3′
138