Table 1 EAE neurological scores
Score | Clinical signs |
|---|---|
0 | No sign of disease; the tail is erect, and locomotor activity is intact |
0.5 | Tail is erect, but the tip is limp |
1.0 | Tail is limp but signs of movement remain. Locomotor activity is slightly impaired and hind legs are extended |
1.5 | Tail is limp and one of two hind legs appears to be weak. Balance is slightly impaired |
2.0 | Tail is limp with no signs of movement; both hind legs are weak. Locomotor activity is impaired, and mice show poor balance and may drag a hind leg. Even in the absence of uncoordinated movement, the mouse presents with inclined head and poor balance |
2.5 | Tail is limp, both hind legs are weak; one hind leg lacks movement or may be dragging |
3.0 | Tail is limp and both hind limbs are completely paralyzed. Locomotor activity is clearly impaired |
3.5 | Tail is limp and both hind limbs are completely paralyzed, partial front leg paralysis. Locomotor activity is clearly impaired. When turned on its side, mouse is unable to right itself |
4.0 | Tail is limp, complete hind leg, and partial front leg paralysis. In cases where the mouse reaches a score 4 for 2 consecutive days, euthanasia is recommended |
4.5 | Complete hind and partial front leg paralysis, no movement around the cage. Mouse is not alert. Euthanasia is recommended |
5.0 | Mouse is dead |