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Table 4 Expression of TLR2, Fpr2, CD3-γ, TCR-ζ chain, CD137, CB1, CB2, and MBP mRNAs in the brainstem and cerebellum of MOG35-55 immunized mice treated with Veh or PEALut

From: A co-ultramicronized palmitoylethanolamide/luteolin composite mitigates clinical score and disease-relevant molecular markers in a mouse model of experimental autoimmune encephalomyelitis

  Brainstem Cerebellum
PID PID
7 14 21 7 14 21
TLR2/GADPH Veh 1.0 ± 0.2 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.4 1.0 ± 0.3
MOG/Veh 0.7 ± 0.2 12.0 ± 2.8°°° 4.6 ± 0.8 0.7 ± 0.0 17.3 ± 7.6°° 16.2 ± 6.6°
MOG/PEALut 1.0 ± 0.3 6.8 ± 1.9* 3.2 ± 0.6 0.8 ± 0.4 2.6 ± 0.7* 9.7 ± 4.8
Fpr2/GADPH Veh 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.4 1.0 ± 0.2 1.0 ± 0.1
MOG/Veh 1.4 ± 0.5 45.6 ± 11.8°°° 8.0 ± 2.8 1.3 ± 0.3 50.7 ± 26.2°° 32.3 ± 13.0
MOG/PEALut 1.6 ± 0.5 24.6 ± 9.1* 5.1 ± 1.4 1.9 ± 0.2 22.6 ± 11.2 17.3 ± 4.1
CD3γ/GADPH CTRL 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.1
MOG/Veh 1.2 ± 0.6 72.7 ± 9.1°°° 44.6 ± 11.4°°° 1.5 ± 0.4 140.4 ± 72.2°° 151.0 ± 40.7°°
MOG/PEALut 1.3 ± 0.3 35.2 ± 14.4** 31.8 ± 8.8 0.9 ± 0.3 31.0 ± 13.8* 105.7 ± 33.9
TCR-ζchain/GADPH CTRL 1.0 ± 0.3 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.4 1.0 ± 0.1 1.0 ± 0.1
MOG/Veh 0.9 ± 0.2 45.1 ± 8.0°°° 19.3 ± 4.6°° 1.4 ± 0.3 10.6 ± 4.5°° 8.5 ± 1.9°
MOG/PEALut 0.8 ± 0.2 19.2 ± 7.5*** 13.1 ± 3.3 1.4 ± 0.2 3.2 ± 1.1* 7.1 ± 2.2
CD137/GADPH Veh 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.2 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.1
MOG/Veh 1.0 ± 0.0 140.7 ± 47.0°°° 11.9 ± 2.6 1.4 ± 0.0 27.9 ± 13.4°°° 7.3 ± 1.4
MOG/PEALut 1.2 ± 0.0 50.9 ± 20.3** 9.6 ± 2.2 1.8 ± 0.4 5.9 ± 1.2** 6.0 ± 1.4
CB2/GADPH Veh 1.0 ± 0.2 1.0 ± 0.2 1.0 ± 0.3 1.0 ± 0.0 1.0 ± 0.3 1.0 ± 0.2
MOG/Veh 1.2 ± 0.1 19.0 ± 5.7°°° 7.4 ± 2.0°° 0.9 ± 0.1 8.1 ± 2.8°°° 7.5 ± 1.9°°
MOG/PEALut 1.5 ± 0.1 6.5 ± 2.5** 6.3 ± 2.3 1.5 ± 0.3 2.9 ± 0.5* 5.7 ± 1.8
CB1/GADPH Veh 1.0 ± 0.2 1.0 ± 0.3 1.0 ± 0.2 1.0 ± 0.5 1.0 ± 0.2 1.0 ± 0.1
MOG/Veh 0.8 ± 0.2 1.1 ± 0.3 1.2 ± 0.3 1.0 ± 0.2 0.9 ± 0.2 1.1 ± 0.1
MOG/PEALut 0.8 ± 0.1 1.3 ± 0.5 1.1 ± 0.3 1.6 ± 0.4 1.1 ± 0.3 0.9 ± 0.1
MBP/GADPH CTRL 1.0 ± 0.2 1.0 ± 0.1 1.0 ± 0.1 1.0 ± 0.0 1.0 ± 0.1 1.0 ± 0.1
MOG/Veh 1.1 ± 0.0 0.9 ± 0.0 0.8 ± 0.0 0.8 ± 0.1 0.8 ± 0.2 1.1 ± 0.1
MOG/PEALut 1.1 ± 0.1 1.0 ± 0.1 1.0 ± 01 1.0 ± 0.1 0.8 ± 0.1 0.9 ± 0.0
  1. Mice were treated with Veh, MOG/Veh, or MOG/PEALut for 7, 14, and 21 days. Veh or PEALut treatments started at 11 PID. TLR2, Fpr2, CD3γ, TCR-ζ chain, CD137, CB1, CB2, and MBP mRNA values are reported as mean ± SEM. n = 6 mice for each group. Data are shown as relative expression of Veh-treated values normalized to 1. n = 6 mice for each group. °p < 0.05, °°p < 0.01, and °°°p < 0.001 compared with the Veh-treated group. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the MOG/Veh