Fig. 7

Malva parviflora hydroalcoholic extract increases microglia accumulation around the Aβ plaques in the cortex and hippocampus of 5XFAD mice. Amyloid plaques were labeled with thioflavin S (green) and microglia with anti-Iba-1 antibodies (red) on cortical a or hippocampal sections (dentate gyrus) b from 5XFAD mice fed with either normal diet (ND) or high-fat diet (HFD) non-treated (Vehicle) or treated with M. parviflora extract (M. parviflora) for 8 months. Representative micrographs are depicted. Images were captured using the Zeiss Axioskop Observer Z1 inverted fluorescence microscope (× 10, scale bar 100 μm). Lower row represents a × 3 digital magnification from boxed areas (scale bar 30 μm). Sketch indicates the analyzed area within the cortex and the hippocampus, respectively