Skip to main content
Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit

Fig. 4

hIgG reduces neutrophil infiltration into the cord at 24 h post-SCI. a The MPO assay was used to detect MPO activity, which is an indirect measure of the neutrophil population in the spinal cord at 24 h post-SCI. Relative to the control buffer and hIgG (0.4 g/kg), high-dose hIgG (2 g/kg) administered at 15 min post-SCI significantly decreased MPO activity at 24 h post-SCI. b Representative fluorescence images demonstrate neutrophil infiltration and the presence of hIgG at the injury center and 2400 μm rostral and caudal in the spinal cord at 24 h post-SCI. Neutrophils are green (PMN) and overlapping with DAPI (blue), while hIgG is red. Scale bar represents 61 μm. c Relative to control buffer and hIgG (0.4 g/kg), stereological counts demonstrate significantly fewer neutrophils in animals treated with hIgG (2 g/kg). Furthermore, there was a significant difference between the animals treated with control buffer and hIgG (0.4 g/kg). One-way ANOVA, Tukey’s post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001). Data are presented as mean ±SEM values

Back to article page