Fig. 8

hIgG (2 g/kg) results in significant tissue preservation at both acute and chronic time points post-SCI. a At 24 h post-SCI, relative to the control buffer and hIgG (0.4 g/kg), hIgG (2 g/kg) significantly decreased the lesion volume in the injured spinal cord. b From top to bottom, representative images of the SCI lesion for animals treated with control buffer, hIgG (0.4 g/kg), hIgG (2 g/kg), and quantified volumes of the lesion are shown. In vivo very high-resolution ultrasound (VHRUS) imaging in B-Mode (11 × 7mm) was used to quantify the acute lesion volume. c At 6 weeks post-SCI, hIgG (2 g/kg) conferred protection by increasing the white and gray matter volumes, while concurrently decreasing the cavity and lesion tissue volumes. Significant improvements were only observed between the control buffer and hIgG (2 g/kg). d Representative 3D reconstructions of the spinal cords from sham and injured rats treated with control buffer, hIgG (0.4 g/kg), and hIgG (2 g/kg). Dark blue indicates white matter, purple indicates gray matter, while lesion and cavity are indicated by yellow and light blue, respectively. Images of LFB/HE-stained spinal cord tissue from all treatment groups and sham, with tissue sections from the epicenter, 960 μm caudal and rostral from the injury site. For VHRUS, one-way ANOVA Tukey’s post-hoc test (*p < 0.05, **:p < 0.01, ***p < 0.001, ****p < 0.0001). For LFB/HE, one-way ANOVA, Tukey’s post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons between the control buffer and hIgG (2 g/kg), ^δp < 0.05, ^δδp < 0.01, ^δδδp < 0.001, ^δδδδp < 0.0001). Data are presented as mean ± SEM values