Table 1 List of primary antibodies used for western blot, concentration, incubation parameters, and purpose

Rabbit anti-rat Iba-1; Wako Laboratory; Chemicals 019-19741

1:1000

Overnight; 4 °C

Resident microglia activation

Rabbit anti-rat MMP-9; Millipore; AB19016

1:3000

Overnight; 4 °C

Blood-spinal cord barrier degradation

Rabbit anti-hIgG; Abcam; AB109849

1:5000

Overnight; 4 °C

Human IgG in the spinal cord

Goat anti-rat occludin; sc-8144

1:500

Overnight; 4 °C

Blood spinal-cord barrier integrity

Mouse anti-rat ZO-1; MABT11

1:250

Overnight; 4 °C

Blood spinal-cord barrier integrity

Rabbit anti-rat VCAM-1; Abcam; AB134047

1:4000

Overnight; 4 °C

Vascular adhesion molecule

Mouse anti-rat β-actin HRP conjugated; ab49900

1:10000

1.5 h; room temperature

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