Fig. 3

CKs and LPS induce opposite changes in microglia morphology. a Representative confocal images comparing Iba1 immunolabeling in untreated (control) and CK- and LPS-treated (4H and 6H) cultures. b Box plots show pooled data from different experiments. Note the significant decrease in total dendrites length and transformation indices upon CKs 6H (left); on the opposite, a significant increase in total dendrites length and in the transformation indices is reported at LPS 6H (right). **P < 0.01, ***P < 0.001, Kruskal-Wallis test (Dunn’s post hoc test). c, d Production of cytokines (TNF-α; IL-4; IL-6; IL-10; INF-γ) and chemokines (CXCL1; CXCL2) determined by Milliplex assay of organotypic culture supernatants after incubation with CKs (c) and LPS (d) for 6H. Bar plot (mean ± SEM of 22 and 14 slices, respectively); **P < 0.01, ***P < 0.001, one-way ANOVA