Fig. 5
Effects of CKs on GABAergic synaptic current duration in the presence of bumetanide. a Sketch of the protocol used to treat slices with bumetanide for 24H (BUM 24H) followed by CKs 4H. b Representative traces (left) and superimposed isolated events (right) of IPSCs in control, after BUM 24H (green), and BUM 24H + CKs 4H (magenta). c The box plot summarizes the IPSCs τ values measured in the three conditions; note the significant reduction in IPSCs τ in BUM 24H and BUM 24H + CKs 4H, in respect to control. Inset: superimposed averaged and scaled IPSCs in the three conditions. *P = 0.012, **P = 0.005, Kruskal-Wallis test. d Top, IPSCs averaged and superimposed traces recorded at different Vh in control (black), BUM 24H (green), and BUM 24H + CKs 4H (magenta). Bottom, I/V curves obtained by plotting GABAA-IPSCs mean amplitude against Vh. Note in the inset the significant differences in the estimated IPSCs reversal potential (EGABA) at BUM 24H and BUM 24H + CKs 4H, when compared to control. *P < 0.05, **P < 0.01, one-way ANOVA