Fig. 3

MGL1 but not MGL2 induces apoptosis of activated mouse T helper cells. Naïve CD4⁺ T cells from wild-type C57Bl/6 mice were stimulated for 3 days with CD3 and CD28 mAbs. Activated cells were washed and left untreated or incubated ON with 10 μg/ml of the indicated Fc-chimeras in the presence (white bars) or absence (black bars) of the Ca²⁺-chelator EDTA (5 mM). Then, cells were washed and stained with 7-AAD and AV and analyzed by flow cytometry. Cell death was determined as the difference in the percentage of AV⁺ cells between polarized cells treated with or without MGL-Fcs. Data are from three independent experiments with cells from three mice. One-way ANOVA followed by Tukey’s multiple comparisons test; *P < 0.05; **P < 0.01; ***P < 0.001