Fig. 5
Increased pro-inflammatory immune response in Clec10a−/− mice. MOG35–55-specific proliferation (a, b) and IL-17 production (c, d) by DLN cells (a, c) and splenocytes (b, d) from WT and Clec10−/− mice 27 days post-immunization (dpi; endpoint of experiments in Fig. 2). Proliferation was assessed by [3H]-thymidine incorporation, and cytokine secretion was analyzed by ELISA after 72 h of MOG35–55 restimulation. Values represent the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. cells from WT mice stimulated with MOG35–55 (Student t test). e Serum anti-MOG35–55 IgG1 and IgG2a antibody titers from WT and Clec10−/− mice obtained at 27 dpi. Mean ± SEM. Mann Whitney U test, *P < 0.05. f IgG1/IgG2c ratio per mouse from the titers shown in e. Mean ± SEM. Mann Whitney U test, **P < 0.01. a–f One representative experiment out of two is shown