Fig. 2

In vitro stimulation of T cells lacking Oct1 results in decreased expression of markers associated with activation and increased expression of markers associated with anergy. a Oct1-deficient and control CD4⁺ T cells stimulated in vitro with indicated antibodies and analyzed by flow cytometry. Representative frequencies of ICOS-expressing CD4⁺ CD44⁺ cells are shown. b Quantification of cells independently purified from the spleens of three mice, with three technical culture replicates for each mouse. c Representative flow cytometry plots showing frequencies of CD25-expressing CD4⁺ CD44⁺ cells in Oct1-deficient and control CD4⁺ T cells. d Quantification from three animals. e Representative expression of CTLA-4 in CD4⁺ CD44⁺ T cells is plotted as histograms for Oct1-deficient and control CD4⁺ T cells. f CTLA4⁺ percentages from three animals, with three culture replicates per animal, are plotted. g Expression of FR4 and CD73 in Oct1-deficient and control CD4⁺CD44⁺ cells. h Averaged percentages of FR4^hiCD73^hiCD4⁺CD44⁺ cells are plotted