Fig. 4

Normal immune responses in Oct1 T cell-deficient mice during JHMV infection. a CD4-Cre;Oct1^fl/fl or Oct1^fl/fl mice were infected i.c. with 200 PFU of JHMV and sacrificed at days 7 (n = 8), 12 (n = 4–5), and 21 (n = 6) p.i. to assess T cell infiltration into the brain. Representative flow analysis depicting CD4⁺ T cell infiltration into brains of mice at day 7 p.i. b Quantification of CD4⁺ T cells as shown by calculating both frequencies and numbers of isolated cells. c Representative flow analysis depicting CD8⁺ T cell infiltration into brains of mice at day 7 p.i. d Quantification of CD8⁺ T cells as shown by calculating both frequencies and numbers of isolated cells. e Representative M133-147 tetramer staining of CD4⁺ T cells from brains of JHMV-infected experimental mice. f Quantification of frequency and numbers of M133-147 tetramer CD4⁺ T cells from experimental groups. g Representative S510-518 tetramer staining of CD8⁺ T cells from brains of JHMV-infected experimental mice. h Quantification of frequency and numbers of M133-147 tetramer CD4⁺ T cells from experimental groups. Data presented are derived from two independent experiments; day 7 p.i., CD4-Cre;Oct1^fl/fl n = 8,Oct1^fl/fl mice n = 8; day 12 p.i., CD4-Cre;Oct1^fl/fl n = 5,Oct1^fl/fl mice n = 4. i IFNγ-producing CD4⁺ (left panel) and CD8⁺ (right panel) CNS-infiltrating T cell percentages are shown for representative animals. j Averaged frequencies (left panel) and total cell numbers (right panel) of CD4⁺ and CD8⁺ cells analyzed as in b. N = 6 for each group