Fig. 1

Chronic WD consumption increased Ccr2::RFP expressing cells in the brain. a–f Representative cortical sections from 12-month-old Cx3cr1^GFP/+Ccr2^RFP/+ mice fed a CD (a–c) or WD (d–f). g, h Magnified images from boxed region shown on c and f, respectively. i The average number of GFP+, GFP+RFP+ (arrows), and GFP+RFP− cells per image (averaged across at least three images, see the “Methods” section) from mice fed a CD or WD (T test was performed in cell counts of each category, *p < 0.05, **p < 0.01). Scale bars, 40 μm