Fig. 2

CCR2 deficiency inhibited the WD-induced increase in IBA1+ cells. a–l Representative cortical sections of IBA1 staining from 12-month-old WT (a–f) and Ccr2-KO mice (g–l) under CD or WD. c, f, i, and l are magnified images from boxed region shown on a, d, g, and j, respectively. m The average number of IBA1+ cells per image (averaged across at least three images) increased in WT mice but not in Ccr2-KO mice (two-way ANOVA followed by Tukey HSD post hoc test, *p < 0.05, **p < 0.01). Scale bars: a, b, d, e, g, h, j, and k, 100 μm; c, f, i, and l, 40 μm