Fig. 3

CCR2 deficiency inhibited the WD-induced increase in CD11b+CD45^hi cells. a–g Gating strategy to isolate CD11b+CD45^lo and CD11b+CD45^hi cells by FACS. Forward scatter (FSC) and side scatter (SSC) were used to remove the cell debris and select single cells (a–c). All live cells, the propidium iodide (PI) negative population, were then selected for immune cell marker profiling (d). CD45+ cells were selected (e) and were subdivided into CD11b–CDe3+ cells and CD11b+CD3e− cells (f). CD11b+CD3e− cells were further subdivided into CD11b+CD45^lo and CD11b+CD45^hi cells (g). Representative gating images were from a 12-month B6 mouse fed a CD. h The percent of CD11b+CD45^lo (g) from total CD11b+CD45+ cells (f). i The percent of CD11b+CD45^hi cells (g) from total CD11b+CD45+ cells (f). Brain samples were from WT or Ccr2-KO mice of 6-month fed a CD, or 12-month fed a CD or WD (two-way ANOVA followed by Tukey HSD post hoc test, *p < 0.05, **p < 0.01)