Fig. 3
From: C3aR signaling and gliosis in response to neurodevelopmental damage in the cerebellum
Morphologies of the P10 Smarca5 cKO and dKO cerebellum. P10 wild-type, C3aR KO, Smarca5 cKO, and dKO cerebellums were labeled to show Purkinje cells (calbindin) and astroglia (GFAP) in sections through the vermis. All sections are oriented with the anterior end towards the top. Large open arrows point to the Purkinje cell layer; small closed arrows point to the external granule cell layer (EGL), and small open arrows point to choroid plexus (ChP). Also labeled is the region of the deep cerebellar nuclei (DCN). Both the Smarca5 cKO and dKO cerebellums displayed more intense GFAP labelling; this labelling in the dKO was more prominent around the periphery. The dKO also displayed a more pronounced loss of EGL in comparison to the Smarca5 cKO single mutant. The C3aR single mutant did not have any apparent abnormalities in comparison to the wild-type sections. The scale bar in the middle panel = 500 μm, and applies to all images