Fig. 4

VGF production in P10 Smarca5 cKO Purkinje cells and Bergmann glia. VGF immunofluorescence labeling generally consisted of diffuse punctae in the cerebellum. Discrete labeling co-localized with calbindin⁺ Purkinje cell dendritic trees (open arrows) of the Smarca5 cKO mutants (a), with similar labeling occurring in the Smarca5;C3aR1 dKO mutants (Additional file 4). Similar labeling was not observed in sections from control animals. Strong VGF labeling was also noted co-localizing with some strongly GFAP⁺ Bergmann glia (open arrows) (b). The closed arrows indicate a weakly GFAP⁺ Bergmann glia cell with only faint VGF labeling. BLBP labeling was used to mark Bergmann glia cell bodies and processes in all samples. Right-hand images show the colors separated from the boxed regions of the left-hand images. Scale bar = 50 μm, and applies to all images