Fig. 7

Iba1⁺ cells in the mutant EGL do not express a characteristic microglial marker. Labeling for P2RY12 in the P10 cerebellum of a dKO mutant animal produced co-labeling only with Iba1⁺ cells inside of the EGL (closed arrows). In some of these Iba1⁺ cells, the P2RY12 labeling was relatively limited (closed arrow, bottom panels); however, in the amoeboid shaped Iba1⁺ cells found in association with the EGL (open arrows), no P2RY12 labeling was detectable. The bottom panels are enlargements of the boxed area indicated in the top panels. Scale bar = 100 μm