Fig. 8

Increased MerTK expression in the Smarca5 cKO cerebellum is attenuated in the absence of C3aR. a qRT-PCR analysis indicated that MerTK was increased over 2-fold in the Smarca5 cKO cerebellum at P10 (n = 5 mice/genotype), but not at P1 (n = 3 mice/genotype). This increase was almost completely attenuated with the loss of C3aR in the dKO mice (*p < 0.05). Transcripts for two other proteins involved in efferocytosis, SR-B1 and MFG-E8 (n = 5 mice/genotype), were not increased in any of the mice. b MerTK expression was observed almost exclusively on Iba1⁺ macrophages (arrowheads) and microglia (open arrows) in the mutant cerebellums. Most other labeling with the MerTK antibody was non-specific blood vessel (BV) labeling. Scale bar = 50 μm, and applies to all panels