Fig. 4

Myeloid cell activation in LysM^Cre/+SOCS3^fl/fl diabetic mice. a Fresh blood were collected from WT and LysM^Cre/+SOCS3^fl/fl mice without or with 3-month diabetes and treated with IL-6 for 40 min. pSTAT3 expression was the examined by flow cytometry. Data showing are mean fluorescent intensity (MFI) of pSTAT3 in circulating leukocytes from WT and LysM^Cre/+SOCS3^fl/fl mice with and without diabetes. Student t test, *p < 0.05. b The percentage of CD11b⁺ cells in SOCS3^fl/fl and LysM^Cre/+ SOCS3^fl/fl mice with and without 3-month diabetes. c, d The mean fluorescent intensity (MFI) of CD62L (c) and LFA (d) in circulating leukocytes in SOCS3^fl/fl and LysM^Cre/+ SOCS3^fl/fl mice with and without diabetes. e Percentage of CD62L⁺ and LFA-1⁺ leukocytes in SOCS3^fl/fl and LysM^Cre/+ SOCS3^fl/fl mice with and without diabetes. n = 4~ 6 per group. Data were presented as mean ± SEM. Two-way ANOVA followed by Bonferroni test. *p < 0.05; **p < 0.01