Fig. 5

Increased leukostasis and avascular capillaries in LysM^Cre/+SOCS3^fl/fl diabetic mice. a–c Retinal leukostasis was evaluated by Con-A perfusion in SOCS3^fl/fl and LysM^Cre/+SOCS3^fl/fl mice with/without 2-months diabetes and examined by confocal microscopy. a, b Representative confocal images of retinal flatmount showing entrapped leukocytes (arrows) in diabetic SOCS3^fl/fl (a) and LysM^Cre/+ SOCS3^fl/fl (b). c Bar figure showing the number of entrapped leukocytes in SOCS3^fl/fl and LysM^Cre/+SOCS3^fl/fl mice. d–f Acellular capillary was examined by confocal microscopy of collagen IV (red) and Isolectin B4 (green) stained retinal flatmounts from in SOCS3^fl/fl and LysM^Cre/+SOCS3^fl/fl mice with/without 6-month diabetes. Representative confocal images of retina flatmount stained with collagen IV (Red) and Isolectin B4 (green) in diabetic SOCS3^fl/fl (d) and LysM^Cre/+SOCS3^fl/fl (e) mice. Acellular capillaries are positive for collagen IV but negative for Isolectin B4 (numbered). f Bar figure showing the quantification of acellular capillaries. Data were presented as mean ± SEM. N > 6 per group. Two-way ANOVA followed by Bonferroni test. CON: non-diabetes, DM: diabetes. *p < 0.05; ***p < 0.001