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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: CCAAT/enhancer-binding protein delta regulates the stemness of glioma stem-like cells through activating PDGFA expression upon inflammatory stimulation

Fig. 5

PDGFAA rescues the glioma stem-like cell feature in U373MG and T98G cells lacking CEBPD under IL-1β treatment. a Images collected from tumor spheroids of U373MG or T98G cells stably expressing shLacZ or shC7 treated with or without 5 ng/mL IL-1β or PDGFAA on day 6. Scale bar, 0.2 mm. b Quantitative analysis of tumor spheroid number in a using ImageJ software. c Western blot analyses conducted with antibodies as indicated using protein lysates from U373MG or T98G spheroid cells stably expressing shLacZ or shC7 treated with or without 5 ng/mL IL-1β or PDGFAA on day 6. Expression of α-tubulin served as the internal control. Cells transduced with shLacZ served as the sham control. (Total data were obtained from three independent experiments, and the data shown here were from one representative assay. All data are expressed as mean ± SEM. Differences among groups were determined with one-way ANOVA followed by Tukey’s multiple comparison test. ***p < 0.001) shLacZ, shβ-galactosidase; shC7, shCEBPD

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