Fig. 6

Demyelination in WT versus TLR2^−/− mice after 5 weeks on cuprizone and 2 weeks on normal chow. WT and TLR2^−/− mice were fed either cuprizone or normal chow for 5 weeks, then all mice were fed normal chow for 2 weeks (“recovery period”). At the end of the 2-week recovery period, myelin status in the corpus callosum was analyzed by EM. a Myelin thickness (g-ratios) of normal (“NL”) chow-fed WT versus TLR2^−/− mice. b Myelin thickness of cuprizone (“Cup”)-fed WT versus TLR2^−/− mice. c Percentage of unmyelinated axons in normal chow-fed WT versus TLR2^−/− mice. d Percentage of unmyelinated axons in cuprizone-fed WT versus TLR2^−/− mice. Data points represent individual mice analyzed as described in the “Methods” section. N = 7 mice per experimental condition. Error bars represent the mean ± SEM. Statistical differences were assessed by Mann-Whitney analysis. ***p < 0.001. For NS comparisons: a NL chow: WT vs TLR2^−/−, p = 0.9656. c NL chow: WT vs TLR2^−/−, p = 0.9636