Fig. 3
Modulatory capacity of MOG mRNA-electroporated tolDC. a IFN-γ secretion by allogeneic splenocytes following stimulation with DC (n = 5). Results are shown as mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001 using two-way ANOVA with Bonferroni post-hoc test. Importantly, no differences in IFN-γ secretion could be detected between tolDC, mock-electroporated tolDC, and mRNA-electroporated tolDC mRNA. N.S. not significant. b Modulation of MOG-specific T cell responses by tolDC following electroporation with MOG mRNA. MOG35–55-reactive splenocytes were cocultured with different conditions of mDC and tolDC in a 10:1 ratio. Following overnight incubation, the number of IFN-γ-secreting T cells was quantified using ELISPOT. Results are shown as mean + standard deviation and groups are compared via a one-way ANOVA with Bonferroni post-hoc test. A sole asterisk (*) indicates the results is statistically significant compared to mDC; +, statistically significant comparisons between groups denoted by solid line; **/++p < 0.01; ***/+++p < 0.001. SFC spot-forming cells, N.S. not significant, extracell. extracellular