| iDC | mDC | tolDC | tolDC mock EP | tolDC mRNA EP | ||
---|---|---|---|---|---|---|---|
A. Immune phenotype | |||||||
Gated on viable cells | CD11c | % positive cells | 45.2 (42.9–53.5) | 42.1 (27.3–56.2) | 73.5 (71.9–76.8)AA,BB | 68.7 (66.0–80.1)A,B | 68.0 (65.2–73.8)A |
CD169 | % positive cells | 12.0 (6.0–23.6) | 22.5 (18.4–28.5) | 57.5 (23.4–70.3)A | 36.4 (20.5–65.7) | 42.5 (19.6–69.5) | |
F4/80 | % positive cells | 24.1 (16.4–31.4) | 17.3 (11.8–27.6) | 76.3 (30.7–82.2)B | 70.4 (39.7–82.5)B | 75.5 (39.7–82.5)A,B | |
Gated on viable CD11c+ cells | CD86 | MFI | 13.9 (9.0–24.9)B | 55.1 (37.3–59.4)A | 21.4 (16.8–26.5) | 37.0 (29.2–52.0) | 60.8 (46.6–71.2)AAA,CC |
Fold change in MFI compared to iDC | – | 3.8 (1.7–4.8) | 1.9 (0.8–2.6)D | 2.3 (1.6–4.5) | 3.3 (2.3–7.1) | ||
MHC-II | MFI | 17.3 (9.3–28.8) | 19.85 (10.6–27.0) | 4.8 (2.1–5.6)A,B | 4.7 (2.3–5.3)A,B | 3.6 (2.5–5.6)A,B | |
Fold change in MFI compared to iDC | – | 1.2 (0.9–1.5) | 0.3 (0.1–0.5)DD,BB | 0.3 (0.1–0.4)B | 0.3 (0.1–0.4)B | ||
CD40 | MFI | 2.5 (1.6–2.8)BBB | 12.8 (11.0–21.3)AAA | 7.5 (5.7–10.1)A | 6.0 (4.7–9.4) | 7.3 (5.2–9.1)A | |
Fold change in MFI compared to iDC | – | 6.0 (4.4–7.8) | 2.8 (2.3–5.0)D,B | 2.9 (2.2–3.6)BB | 3.2 (2.8–3.9)B | ||
B. Cytokine secretion profile | |||||||
IL-12p70 (pg/mL) | 2.3 (1.5–5.8)BBB | 356.3 (193.2–409.6)AAA | 73.7 (61.9–132.9)BBB | 74.3 (43.2–98.4)BBB | 99.0 (48.1–122.3)BBB |