Fig. 4

Mdivi-1 reduced the number and percentage of Th1 and Th17 cells in the CNS. Lumbar regions of spinal cords were harvested from mice described in Fig. 1a. a Representative microphotographs for infiltration of CD4⁺ IFN-γ⁺ cells in spinal cords and quantitative analysis of number of CD4⁺ IFN-γ⁺ cells in spinal cords. b Flow cytometry analysis of CD4⁺ IFN-γ⁺ cells in brain and spinal cords and quantitative analysis of percentage of IFN-γ⁺ cells in gated CD4⁺ cells described in Fig. 2c. c Representative microphotographs for infiltration of CD4⁺IL-17⁺ cells in spinal cords and quantitative analysis of number of IL-17⁺ cells in spinal cords. d Flow cytometry analysis of CD4⁺ IL-17⁺ cells in brain and spinal cords and quantitative analysis of percentage of IL-17⁺ cells in gated CD4⁺ cells described in Fig. 2c. Data represent mean ± SEM (n = 5–8 each group). *P < 0.05, ***P < 0.001. One representative of two experiments is shown