Fig. 5

Mdivi-1 increases Treg cell number in the CNS. Lumbar regions of spinal cords were harvested from mice described in Fig. 1a. a Representative microphotographs for infiltration of CD4⁺ IL-10⁺ cells in spinal cords and absolute and/or relative quantitative analysis of number of CD4⁺ IL-10⁺ and CD4⁻ IL-10⁺ cells in spinal cord slices. b Flow cytometry analysis of CD4⁺Foxp3⁺ cells in brain and spinal cords and quantitative analysis of percentage of Foxp3⁺ cells in gated CD4⁺ cells described in Fig. 2c. Data represent mean ± SEM (n = 5–8 each group). *P < 0.05; **P < 0.005. One representative of two experiments is shown