Fig. 1

Identification/characterization of microglia and infiltrating macrophages within the brains of TMEV-infected and PBS-injected mice. a Representative flow cytometry plots showing microglia (CD45^lo/int CD11b⁺), macrophages (CD45^hi CD11b⁺), and lymphocytes (CD45⁺ CD11b^lo/int) isolated from the brains of PBS-injected and TMEV-infected mice at 7 days post infection (d.p.i.). b Quantification of macrophage infiltration into the brains of TMEV-infected (n = 15) and PBS-injected (n = 4) mice at 7 d.p.i. Data represents the mean ± standard error of the mean (SEM). c Major histocompatibility complex class-II (MHC-II) expression on microglia and macrophages at 7 d.p.i. Gates were set according to FMO as described in the “Methods” section. Student’s t test, **p < 0.01, degrees of freedom (df) = 3