Fig. 6

TREM-1 is expressed by infiltrating macrophages. C57BL/6 J mice were intracerebrally infected with TMEV or PBS-injected and mice were euthanized at 3 d.p.i. a Cells were isolated and microglia (CD45^lo/int CD11b⁺), macrophages (CD45^hi CD11b⁺), and lymphocytes (CD45⁺ CD11b^lo/int) were identified by flow cytometry. b Schematic flow plot of TREM-1 expressing cells in PBS-injected and TMEV-infected brains. c Overlay of manually gated microglia, macrophages, lymphocytes, and TREM-1-expressing cells, demonstrating TREM-1 is exclusively expressed in infiltrating macrophages. d Quantification of macrophages (CD45^hi CD11b⁺) in the brains of TMEV-infected mice at 3 and 7 d.p.i. e Percentage of TREM-1-expressing cells in the brains of TMEV-infected mice at 3 and 7 d.p.i. f Quantification of TREM-1-expressing macrophages in the brains of TMEV-infected mice at 3 and 7 d.p.i. d–f n = 6 mice per group; Student’s t test, *p < 0.05,**p < 0.01, df = 5. Gates were set according to FMO as described in the “Methods” section