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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The retinal environment induces microglia-like properties in recruited myeloid cells

Fig. 1

Repopulation of CD11b+YFP+ cells in ocular nervous tissue after tamoxifen-induced DTA ablation of CX3CR1+ cells. CD11cDTR/GFP:CX3CR1YFP-creER:ROSADTA mice were given 2.5 mg tamoxifen on three alternating days and analyzed at the indicated time after the last tamoxifen injection. a Sections through the posterior pole showing peripapillary retina, optic nerve head (OHN), and optic nerve (ON) of control mice (naïve, no tamoxifen) and mice at days 3 and 10 post-tamoxifen. b Flat mounts showing myeloid cells in the indicated layers of peripapillary retina from control mice and mice at days 3 and 10 post-tamoxifen. Layers include the retinal ganglion cells (RGC), the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). White arrows identify the optic nerve head. White scale bars are 100 μm. CD11b—red; GFP—green; YFP—yellow; DAPI—blue. Note that the somas of the YFPhi cells are very bright and often bleed into the GFP channel. This is illustrated by the identification of two cells in the outer plexiform layer of panel B; one is GFPhiYFPhi and the other is GFPloYFPhi (see also Fig. 3a). This is not an issue in flow cytometry which clearly distinguishes YFP and GFP

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