Fig. 8
From: The retinal environment induces microglia-like properties in recruited myeloid cells
Analysis of GFPhi cells in chimeric mice and after diphtheria toxin ablation. a Analysis of retinal GFPhi cells in CD11cDTR/GFP bone marrow (BM) into B6 recipients with or without ONC (O). Time of harvest (H) is indicated. b Survival of host retinal GFPhi cells in CD11cDTR/GFP recipients of B6 bone marrow. c Diphtheria toxin (DTx) treatment ablates only GFPhi microglia. Mice were given a unilateral ONC and then that eye was treated with diphtheria toxin on the indicated days (T) then harvested. Contralateral eyes from the mice (no ONC, no diphtheria toxin) were harvested as controls. Chimeric mice received 2 × 6 Gy of radiation and were unshielded. Cell counts were obtained by staining retinal flat mounts for CD11b and then counting GFPhi or GFPlo cells. The average number of CD11b+GFPhi or CD11b+GFPlo cells (± 1 SD) in a normal CD11cDTR/GFP mouse retina is indicated on the figures by the gray-shaded zone