Fig. 5
From: A detrimental role of RelB in mature oligodendrocytes during experimental acute encephalomyelitis
Deletion of RelB in oligodendrocytes prevents loss of mature oligodendrocytes and demyelination. EAE was induced in WT (RelBloxP/loxP) and RelBΔOLIGO mice. Tissues were collected at the peak of disease. a, e Expression was analyzed by qPCR in lumbar spine tissue. n = 8–14 mice per group. T tests: IL-10 (F = 4.243, p = 0.025); IFNg (F = 1.384, p = 0.032); MBP (F = 22.57, p = 0.03); PLP (F = 5.116, p = 0.04). b, c Immunofluorescence of lumbar spinal cord sections stained with anti-IBA1 (b) and anti-GFAP (c) antibodies. Sections were counterstained with Hoechst. d Lumbar spinal cord sections stained with fluoromyelin and counterstained with Hoechst. f Immunofluorescence of lumbar spinal cord sections stained with anti-CC1. Quantification of CC1 positive cells (IF). Images were taken at least two regions from three different animals per genotype. Percentages of CC1-positive cells to nuclei labeled with Hoechst. Measurements were calculated manually using the cell counter tool in ImageJ (n = 6–7 per group, T test; (F = 1.846, p = 0.04)