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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Depletion of regulatory T cells increases T cell brain infiltration, reactive astrogliosis, and interferon-γ gene expression in acute experimental traumatic brain injury

Fig. 1

CD3+ T cells infiltrate the injured brain tissue in acute experimental TBI. a Scheme illustrating the brain tissue regions examined by qRT-PCR (green box, compared to corresponding regions of naive brains) or immunohistochemistry (IHC, red boxes). b qRT-PCR time course analysis of Cd247 expression in the injured, ipsilesional brain tissue reveals peak expression at 5 dpi. c, d Double-immunostaining using anti-CD3 (green, pan T cell marker) and anti-NeuN (red, pan neuron marker), and DAPI staining (blue, nuclei). c CD3+ T cells were absent in the non-injured, contralesional hemisphere. d CD3+ T cells infiltrated the injured, ipsilesional brain tissue. Brain sections from five mice were examined by IHC at 5 dpi. Data are expressed as mean ± SEM (n = 9–10 per time point) and statistical significance was calculated by one-way ANOVA followed by Dunn’s multiple comparison test (**p < 0.01; ***p < 0.001; ****p < 0.0001; #p < 0.05). Scales: 100 μm, 20 μm (c, d)

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