Fig. 5

Depletion of microglia does not affect the butyrate-induced suppression of demyelination and enhancement of remyelination. a MBP, NF200, and Iba-1 immunocytochemical staining of PLX3397-treated or non-treated organotypic cerebellar slice cultures. Scale bar, 100 μm. b Schematic showing the experimental protocol of demyelination analysis. c Immunocytochemical staining of PLX3397-treated or non-treated organotypic cerebellar slice cultures with LPC and butyrate treatment. Scale bar, 100 μm. d Myelination index (MI) of organotypic cerebellar slice cultures (n = 12 slices per group, pooled from three independent experiments). e Schematic showing the experimental protocol of remyelination analysis. f Immunocytochemical staining of PLX3397-treated or non-treated organotypic cerebellar slice cultures with LPC and butyrate treatment. Scale bar, 100 μm. g MI of organotypic cerebellar slice cultures (n = 12 slices per group, pooled from three independent experiments). h relative expression of iNOS and Arg1 in organotypic slice cultures (n = 5 slice per group). The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001