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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Beneficial effects of curtailing immune susceptibility in an Alzheimer’s disease model

Fig. 4

Effects of the treatment with an anti-TNFSF10 monoclonal antibody on the expression of GITR and FoxP3, as well as of the anti-inflammatory protein IL-10 in the hippocampus of 3xTg-AD mice Panel a: Left: Western blot analysis of GITR, FoxP3, and IL-10 protein expression in the mice following chronic treatment (12 months) with an anti-TNFSF10 monoclonal antibody (10 μg/animal twice a month, i.p.) or vehicle (10 μg/animal twice a month, i.p.). Right: Densitometric analysis of Western blots. *p < 0.05 vs untreated 3xTg-AD mice; **p < 0.05 vs. all other matching groups (one-way ANOVA, followed by a Duncan’s multiple range test). Vertical bars are means ± S.E.M. Panel b: representative immunofluorescence images of hippocampi for GITR (red) and FoxP3 (green) expression and co-localization from the same animal groups as above (white arrows; merge column; DAPI = nuclear staining). Panel c: immunofluorescence by confocal microscopy of hippocampi for IL-10 (red; white arrows) and FoxP3 (green; white arrows) expression and co-localization from the same animal groups as above (merge column; DAPI = nuclear staining). WT wild-type animals (n = 5/group); AD: 3xTg-AD animals (n = 5/group); the respective side columns are the lower magnification samples where the areas to analyze (CA1, CA2, CA3, and CA4) were magnified (framed in a green box)

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