Fig. 7
From: Beneficial effects of curtailing immune susceptibility in an Alzheimer’s disease model
Confocal microscopy for detection of TNF-α and CD11b expression in the microglia of the CA2-CA3 regions of the hippocampus of 3xTg AD mice, following chronic treatment (12 months) with an anti-TNFSF10 monoclonal antibody (10 μg/animal twice a month, i.p.; (n = 10/group) or vehicle (10 μg/animal twice a month, i.p.). Representative images showing respectively CD11b (red, panels A, D, G, L), TNF-α (green, panels B, E, H, M), and TNF-α co-localized with CD11b (panels C, F, I, N). Side graphic represents the densitometric count of positive cells; *p ≤ 0.05 vs. all other groups; (two-way ANOVA, followed by Bonferroni post-hoc test). Vertical bars are means ± S.E.M. WT wild-type animals (n = 5/group); AD: 3xTg-AD animals (n = 5/group). Black arrows point resting microglia; white arrows point activated microglia (panels G and I), or co-localization TNF-α/CD11b (panels I and N)