Fig. 10
LPA1 antagonism attenuates MAPK phosphorylation and enhances Akt phosphorylation in the ischemic brain after tMCAO challenge. Mice were challenged with tMCAO, and AM095 (30 mg/kg, p.o.) was administered immediately after reperfusion. Total protein was extracted from the ipsilateral brain hemisphere at 1 day after tMCAO challenge. Phosphorylation levels of MAPKs and Akt were then determined by Western blot analysis. Representative Western blots (a) and their quantification (b). n = 4 mice per group. *p < 0.05 and **p < 0.01 versus sham. #p < 0.05 and ##p < 0.01 versus vehicle-administered tMCAO mice (tMCAO+veh)