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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Lysophosphatidic acid receptor 1 (LPA1) plays critical roles in microglial activation and brain damage after transient focal cerebral ischemia

Fig. 5

LPA1 antagonism reduces microglial activation in the ischemic brain at 3 days after tMCAO challenge. Mice were challenged with tMCAO, and AM095 (30 mg/kg, p.o.) was administered immediately after reperfusion. Microglial activation was assessed at 3 days after tMCAO challenge by Iba1 immunohistochemistry. a Representative images of Iba1-immunopositive cells in the periischemic (P) and the ischemic core (C) regions. Diagram boxes display the cerebral area where images in middle and bottom panels are acquired. Scale bars, 200 μm (top panels) and 50 μm (middle and bottom panels). Open arrowheads indicate ramified microglia, and closed arrowheads indicate amoeboid microglia. b Quantification of the number of Iba1-immunopositive cells in both regions. c Quantification of soma size of Iba1-immunopositive cells in both regions. d Quantification of the number of morphologically transferred microglial cells in the ischemic core region (ramified microglia to amoeboid microglia transformation). n = 5 (sham), 5 (tMCAO+veh), and 4 (tMCAO+AM095). ***p < 0.001 versus sham. ##p < 0.01 and ###p < 0.001 versus vehicle-administered tMCAO mice (tMCAO+veh)

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